IKKγ (H81) polyclonal antibody

Product Specifications

Background

Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase α (IKKα), previously designated CHUK, interacts with IkB-α and specifically phosphorylates I˚Bα on Serine 32 and 36, the sites that trigger its degradation. IKKα appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKα is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

NF-kappa-B essential modulator; NEMO; FIP-3; IkB kinase-associated protein 1; IKKAP1; Inhibitor of nuclear factor kappa-B kinase subunit gamma; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma; NF-kappa-B essential modifier; IKBKG; FIP3; NEMO; IKK-γ

Swiss Prot

Q9Y6K9

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 50-100 of Human IKKγ.

Conjugation

Unconjugated

Related Pathways

NF-kB Signaling, STING Signaling, Inhibition of Apoptosis, Toll-like Receptor signaling

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 42 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IKKγ (H81) polyclonal antibody detects endogenous levels of IKKγ protein.

Applications Notes

Western blot (WB) analysis of IKBKG pAb at 1:500 dilution Lane1:MEF whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:H1792 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:Panc1 whole cell lysate (40ug)