Myc (K422) polyclonal antibody

Product Specifications

Background

C-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64; MYC; BHLHE39

Swiss Prot

P01106

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 401-450 of Human Myc.

Conjugation

Unconjugated

Related Pathways

Warburg Effect, Signaling Pathways Activating p38 MAP KinaseCell Cycle Control G1 S Checkpoint, Glutamine Metabolism

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Myc (K422) polyclonal antibody detects endogenous levels of proto-oncogene c-Myc protein.

Applications Notes

Western blot (WB) analysis of MYC pAb at 1:1000 dilution Lane1:3T3-L1 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)