VDR (N204) polyclonal antibody

Product Specifications

Background

The vitamin D receptor (VDR), also known as the calcitriol receptor, and also known as NR1I1 (nuclear receptor subfamily 1, group I, member 1), is a member of the nuclear receptor family of transcription factors. Upon activation by vitamin D, the VDR forms a heterodimer with the retinoid-X receptor and binds to hormone response elements on DNA resulting in expression or trans-repression of specific gene products.It is an intracellular hormone receptor that specifically binds 1,25 (OH) 2D3 and mediates its effects. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Vitamin D3 receptor; VDR; 1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1; NR1I1

Swiss Prot

P11473

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 161-210 of Human VDR.

Conjugation

Unconjugated

Related Pathways

Nuclear Receptor Signaling

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 43, 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

VDR (N204) polyclonal antibody detects endogenous levels of VDR protein

Applications Notes

Western blot (WB) analysis of VDR (N204) pAb at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:3T3-L1 whole cell lysate (40ug) Lane4:The Kidney tissue lysate of Rat (40ug)