P-H2BFS (K5) polyclonal antibody

Product Specifications

Background

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Histone H2B type F-S; Histone H2B.s; H2B/s; H2BFS

Swiss Prot

P57053

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to Human H2BFS (phospho-K5) .

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 17kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

H2BFS (phospho-K5) polyclonal antibody detects endogenous levels of H2BFS (phospho-K5) protein.

Applications Notes

Western blot (WB) analysis of H2BFS (phospho-K5) polyclonal antibody at 1:500 dilution Lane1:SHSY5Y whole cell lysate (40ug) Lane2:HuT78 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:Raw264.7 whole cell lysate (40ug) Lane5:H9C2 whole cell lysate (40ug) Lane6:The spleen tissue lysate of Mouse (40ug)