ATP1A2 polyclonal antibody
Product Specifications
Background
The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Sodium/potassium-transporting ATPase subunit alpha-2; Na (+) /K (+) ATPase alpha-2 subunit; Sodium pump subunit alpha-2; ATP1A2; KIAA0778
Swiss Prot
P50993
Reactivity
Human, Mouse
Immunogen
Synthetic peptide, corresponding to Human ATP1A2 .
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 95kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ATP1A2 polyclonal antibody detects endogenous levels of ATP1A2 protein.
Applications Notes
Western blot (WB) analysis of ATP1A2 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:H1792 whole cell lysate (40ug) Lane3:Panc1 whole cell lysate (40ug) Lane4:Aml-12 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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