RTCD1 polyclonal antibody

Product Specifications

Background

RTCD1 catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. There are two named isoforms.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

RNA 3'-terminal phosphate cyclase; RNA cyclase; RNA-3'-phosphate cyclase; RNA terminal phosphate cyclase domain-containing protein 1; RTC domain-containing protein 1; RTCA; RPC; RPC1; RTC1

Swiss Prot

O00442

Reactivity

Human, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 321-366 of Human RTCD1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 43 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RTCD1 polyclonal antibody detects endogenous levels of RTCD1 protein.

Applications Notes

Western blot (WB) analysis of RTCA pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug)