E2F-6 (E170) polyclonal antibody

Product Specifications

Background

The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcriptional regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Transcription factor E2F6; E2F-6; E2F6

Swiss Prot

O75461

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 141-190 of Human E2F-6.

Conjugation

Unconjugated

Related Pathways

Cell Cycle Control G1 S Checkpoint

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

E2F6 (E170) polyclonal antibody detects endogenous levels of E2F6 protein.

Applications Notes

Western blot (WB) analysis of E2F6 pAb at 1:500 dilution Lane1:A2780 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug)