Caspase 2 (K152) polyclonal antibody

Caspase-2 (Nedd2, ICH-1) is an aspartate-specific cysteine protease that is activated in response to various apoptotic stimuli. Caspase-2 is unique among the caspases in that it has features of both upstream caspases (long prodomain) and downstream caspases (DEXD substrate specificity) . Caspase-2 is highly expressed in the brain during development and is expressed at low levels in adult tissue. Specifically, caspase-2 localizes to the mitochondria, the Golgi, the cytoplasm and the nucleus. Caspase-2 exists as two isoforms, caspase-2L and caspase-2S, which are produced by alternative splicing and differ in their N- and C-termini. Caspase-2L acts as a positive regulator of apoptosis, whereas caspase-2S functions as a negative regulator of apoptosis. Following apoptotic stimuli, the caspase-2L precursor undergoes cleavage at Asp 153 to produce a fragment (p30) . The p30 fragment undergoes further cleavage to generate a fragment containing amino acids 153-308 (p18) and a fragment containing amino acids 317-435 (p13 or p14) . As apoptosis progresses, the p13 (p14) fragment can undergo further processing to yield a fragment containing amino acids 331-435 (p12) .

Caspase-2; Caspase 2; Caspase2; CASP-2; Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2; Protease ICH-1; CASP2; ICH1; NEDD2

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of CASP2 pAb at 1:500 dilution Lane1:Jurkat whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug)