Claudin 1 (R197) polyclonal antibody
Product Specifications
Background
The claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and junction adhesion molecule (JAM) . Claudins, which consist of four transmembrane domains and two extracellular loops, make up tigh junction strands. Emerging evidence suggests that the claudin family of proteins regulates transport through tight junctions via differential discrimination for solute size and charge. Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Claudin-1; CLD1; ILVASC; SEMP1; Claudin1; CLDN1; UNQ481/PRO944
Swiss Prot
O95832
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic peptide, corresponding to amino acids 171-220 of Human Claudin-1.
Conjugation
Unconjugated
Related Pathways
Contribution of ECM and Cytoskeletal Factors to EMT
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 22 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Claudin 1 (R197) polyclonal antibody detects endogenous levels of Claudin 1 protein.
Applications Notes
Western blot (WB) analysis of Claudin 1 (R197) pAb at 1:500 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:The Liver tissue lysate of Mouse (40ug) Lane3:The Kidney tissue lysate of Rat (20ug) Lane4:L02 whole cell lysate (40ug) Lane5:H1792 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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