Podocin polyclonal antibody
Product Specifications
Background
The pathological conditions of SRN1 correlate well with mutations at the NPHS2 gene, where expression of a protein known as Podocin occurs. Abnormal or inefficient signaling through Podocin protein-dependent networks contributes to the development of podocyte dysfunction and proteinuria. The human NPHS2 gene maps to chromosome 1q25-q31 and encodes a 383 amino acid protein. Podocin is an integral membrane protein that appears to fold into a hairpin-like structure with intracellular amino- and carboxy-termini. Transmembrane and cytoplasmic portions of Podocin share homology to the corresponding regions of the stomatin family proteins. Expression of high-order oligomers of Podocin in glomerular podocytes may reflect a scaffolding function that influences proper function of the glomerular filtration barrier, which is necessary for renal stability.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
NPHS2
Swiss Prot
Q9NP85
Reactivity
Rat, Mouse
Immunogen
A synthetic peptide corresponding to residues in Human Podocin.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 42 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Podocin polyclonal antibody detects endogenous levels of Podocin protein.
Applications Notes
Western blot (WB) analysis of Podocin pAb at 1:1000 dilution Lane1:The Kidney tissue lysate of Mouse (20ug) Lane2:The Kidney tissue lysate of Rat (30ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS6204084-01 0.1 mL
VPS8 (KIAA0804, Vacuolar Protein Sorting-associated Protein 8 Homolog) (MaxLight 490)
Ask
View Details MBS6204084-02 5x 0.1 mL
VPS8 (KIAA0804, Vacuolar Protein Sorting-associated Protein 8 Homolog) (MaxLight 490)
Ask
View Details 
