RalBP1 polyclonal antibody
Product Specifications
Background
The RalA binding protein 1 (RalBP1 or RLIP76) was originally identified as a GTP-RalA associated protein that acted as a downstream RalA effector in regulating Ral-Ras signaling. RalBP1 interacts with RalA and the endocytosis protein REPS2 (POB1) through its carboxy-terminal Ral binding domain. RalBP1 has an intrinsic GTPase activating function and interacts with Cdc42 through its centrally located Rho-GAP domain. A protein complex containing RalBP1/POB1/RalA regulates endocytosis of membrane receptors. RalBP1 also functions as a non-ABC transporter that catalyzes the ATP-dependent transport of numerous xenobiotics, including glutathione conjugates and some chemotherapeutic agents. RalBP1 transporter activity may play an important role in detoxification, drug resistance and the stress response. Increased expression of RalBP1 protein is associated with some forms of cancer and regression of cancer xenografts results from RalBP1 inhibition. Evidence to date suggests that RalBP1 may be a promising therapeutic target for cancer therapy.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
RalA-binding protein 1; RalBP1; 76 kDa Ral-interacting protein; Dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; Ral-interacting protein 1; RALBP1; RLIP1; RLIP76
Swiss Prot
Q15311
Reactivity
Human, Rat, Mouse
Immunogen
Recombinant protein of human RalBP1.
Conjugation
Unconjugated
Applications
WB IP
Dilution
WB: 1:5000~1:10000 IP:1:2000~1:8000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 100 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
RalBP1 polyclonal antibody detects endogenous levels of RalBP1 protein.
Applications Notes
Western blot (WB) analysis of RalBP1 polyclonal antibody at 1:5000 dilution Lane1:CT26 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:Jurkat whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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