Endonuclease G polyclonal antibody

Product Specifications

Background

Endonuclease G (EndoG) is a nuclear-encoded mitochondrial nuclease that has been reported to function in apoptosis, DNA recombination and cell proliferation. EndoG is expressed as a precursor protein containing an amino-terminal mitochondrial targeting sequence which is removed when the protein is imported into the mitochondria. During apoptosis EndoG is released from the mitochondria, translocates to the nucleus and cooperates with other nucleases to trigger DNA fragmentation associated with the apoptotic process

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Product Name Alternative

Endonuclease G, mitochondrial ; Endo G; ENDOG

Swiss Prot

Q14249

Reactivity

Human, Rat, Mouse

Immunogen

Recombinant protein of human Endonuclease G.

Conjugation

Unconjugated

Applications

WB IP IHC

Dilution

WB: 1:2000~1:5000 IP:1:2000~8000 IHC:1:50~1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 30 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Endonuclease G polyclonal antibody detects endogenous levels of Endonuclease G protein.

Applications Notes

Western blot (WB) analysis of Endonuclease G polyclonal antibody at 1:5000 dilution Lane1:K562 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:The Heart tissue lysate of Mouse (40ug) Lane5:The Muscle tissue lysate of RAT (40ug)