Endonuclease G polyclonal antibody
Product Specifications
Background
Endonuclease G (EndoG) is a nuclear-encoded mitochondrial nuclease that has been reported to function in apoptosis, DNA recombination and cell proliferation. EndoG is expressed as a precursor protein containing an amino-terminal mitochondrial targeting sequence which is removed when the protein is imported into the mitochondria. During apoptosis EndoG is released from the mitochondria, translocates to the nucleus and cooperates with other nucleases to trigger DNA fragmentation associated with the apoptotic process
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.
Product Name Alternative
Endonuclease G, mitochondrial ; Endo G; ENDOG
Swiss Prot
Q14249
Reactivity
Human, Rat, Mouse
Immunogen
Recombinant protein of human Endonuclease G.
Conjugation
Unconjugated
Applications
WB IP IHC
Dilution
WB: 1:2000~1:5000 IP:1:2000~8000 IHC:1:50~1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 30 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Endonuclease G polyclonal antibody detects endogenous levels of Endonuclease G protein.
Applications Notes
Western blot (WB) analysis of Endonuclease G polyclonal antibody at 1:5000 dilution Lane1:K562 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:The Heart tissue lysate of Mouse (40ug) Lane5:The Muscle tissue lysate of RAT (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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