BID polyclonal antibody

Product Specifications

Background

Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax. Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane. Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation. Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Product Name Alternative

BH3-interacting domain death agonist; p22 BID; BID; p15 BID; p13 BID; p11 BID

Swiss Prot

P55957

Reactivity

Human

Immunogen

Recombinant protein of human BID.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis, Death Receptor Signaling

Applications

WB IP IHC

Dilution

WB: 1:2000~1:5000 IP:1:2000~1:8000 IHC:1:50~1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 25 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

BID polyclonal antibody detects endogenous levels of BID protein.

Applications Notes

Western blot (WB) analysis of BID polyclonal antibody at 1:5000 dilution Lane1:Jurkat whole cell lysate (40ug) Lane2:THP-1 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug)