EIF1 polyclonal antibody

Product Specifications

Background

In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors. Eukaryotic translation initiation factor 1 (eIF1) is crucial for the scanning process in vitro. During the scanning process, eIF1 is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of eIF1 in regulating the activity of ribosomal subunits 43S, 48S and 40S. eIF1 enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. It is also a promotor, along with eukar-yotic translation initiation factor 1A (eIF1A), for assembly of 48S ribosomal complexes at the initiation codon of a conventional capped mRNA. In addition, eIF1 and eIF1A, together with eukaryotic translation initiation factor 5 (eIF5), function in the formation of stable 40S ribosomal preinitiation complexes.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Eukaryotic translation initiation factor 1; eIF1; A121; Protein translation factor SUI1 homolog Sui1iso1; EIF1; SUI1

Swiss Prot

P41567

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human EIF1.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 13 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

EIF1 polyclonal antibody detects endogenous levels of EIF1 protein.

Applications Notes

Western blot (WB) analysis of EIF1 pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:Raw264.7 whole cell lysate (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:THP-1 whole cell lysate (40ug) Lane5:PC3 whole cell lysate (40ug) Lane6:The lung tissue lysate of Rat (40ug) Lane7:The spleen tissue lysate of Mouse (40ug)