Cdc25C (phospho-S216) polyclonal antibody
Product Specifications
Background
Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C; CDC25C
Swiss Prot
P30307
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Cdc25C around the phosphorylation site of Serine 216.
Conjugation
Unconjugated
Related Pathways
G Protein-coupled Receptors Signaling to MAPK Erk, Cell Cycle G2 M DNA Damage Signaling Pathway
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-CDC25C (S216) polyclonal antibody detects endogenous levels of CDC25C protein only when phosphorylated at Ser216.
Applications Notes
Western blot (WB) analysis of p-CDC25C (S216) pAb at 1:500 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:CT-26 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit
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