Tensin 1 (Phospho-Y1326) polyclonal antibody

Product Specifications

Background

The protein encoded by this gene localizes to focal adhesions, regions of the plasma membrane where the cell attaches to the extracellular matrix. This protein crosslinks actin filaments and contains a Src homology 2 (SH2) domain, which is often found in molecules involved in signal transduction. This protein is a substrate of calpain II. Alternative splicing results in multiple transcript variants encoding different isoforms.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Product Name Alternative

Tensin-1; TNS1; TNS

Swiss Prot

Q9HBL0

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic phosphopeptide derived from human Tensin 1 around the phosphorylation site of Tyrosine 1326.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 200 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Tensin 1 (Phospho-Y1326) polyclonal antibody detects endogenous levels of Tensin 1 protein only when phosphorylated at Tyr1326.

Applications Notes

Western blot (WB) analysis of Tensin 1 (Phospho-Y1326) polyclonal antibody at 1:500 dilution LaneA:MEF whole cell lysate (40ug) LaneB:PC12 whole cell lysate (40ug) LaneC:LOVO whole cell lysate (40ug)