RAB20 polyclonal antibody

Product Specifications

Background

The Ras-related superfamily of guanine nucleotide binding proteins includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies all of which are thought to play an important role in either endocytosis or in biosynthetic protein transport. The process of transporting newly synthesized proteins from the endoplasmic reticulum (ER) to various stacks of the Golgi complex and to secretory vesicles involves the movement of carrier vesicles and requires Rab protein function. Rab proteins are also an integral part of endocytic pathways. Rab 20 (Ras-related protein Rab-20) is a 234 amino acid protein that localizes to the golgi apparatus and belongs to the Rab subfamily of small GTPases. Expressed in normal and cancerous pancreatic tissue, Rab 20 plays an important role in apical endocytosis and recycling and may be involved in the development of exocrine pancreatic adenocarcinomas.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Product Name Alternative

Ras-related protein Rab-20; RAB20

Swiss Prot

Q9NX57

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human RAB20.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 26 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RAB20 polyclonal antibody detects endogenous levels of RAB20 protein.

Applications Notes

Western blot (WB) analysis of RAB20 polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:H1792 whole cell lysate LaneC:BV2 whole cell lysate LaneD:MEF whole cell lysate LaneE:C6 whole cell lysate