Rabaptin 5 beta polyclonal antibody
Product Specifications
Background
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas9) system is an adaptive immune response defense mechanism used by archea and bacteria for the degradation of foreign genetic material. This mechanism can be repurposed for other functions, including genomic engineering for mammalian systems, such as gene knockout (KO) and gene activation. CRISPR Activation Plasmid products enable the identification and upregulation of specific genes by utilizing a D10A and N863A deactivated Cas9 (dCas9) nuclease fused to a VP64 activation domain, in conjunction with sgRNA (MS2), a target-specific sgRNA engineered to bind the MS2-P65-HSF1 fusion protein. This synergistic activation mediator (SAM) transcription activation system provides a robust system to maximize the activation of endogenous gene expression.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.
Product Name Alternative
Rab GTPase-binding effector protein 2; Rabaptin-5beta; RABEP2; RABPT5B
Swiss Prot
Q9H5N1
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic peptide, corresponding to Human Rabaptin 5 beta.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB:1:500~1:1000 IHC:1:50~1:200
Purity
The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 72 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Rabaptin 5 beta polyclonal antibody detects endogenous levels of Rabaptin 5 beta protein.
Applications Notes
Western blot (WB) analysis of Rabaptin 5 beta polyclonal antibody at 1:500 dilution LaneA:BV2 whole cell lysate LaneB:PC12 whole cell lysate LaneC:A549 whole cell lysate LaneD:LOVO whole cell lysate
Host or Source
Rabbit
Available Sizes
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