MYPT3 polyclonal antibody

Product Specifications

Background

The members of the MYPT family, MYPT1, MYPT2 and MYPT3 are the myosinbinding subunits of myosin phosphatase and an integral component of the myosin protein phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. MYPT1 is localized on stress fibers and is distributed close to the cell membrane and at cell-cell contacts to regulate myosin phosphatase activity. In addition to MYPT1, a novel isoform of MYPT1, MYPT2, also interacts with PPlc. MYPT3, also designated PP16A, inhibits protein phosphatase activity involving phosphorylase, myosin light chain and myosin substrates. It acts as a lipid anchor and binds PP1. MYPT3 localizes primarily to the cell membrane.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Product Name Alternative

Protein phosphatase 1 regulatory subunit 16A; Myosin phosphatase-targeting subunit 3; PPP1R16A; MYPT3

Swiss Prot

Q96I34

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human MYPT3.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MYPT3 polyclonal antibody detects endogenous levels of MYPT3 protein.

Applications Notes

Western blot (WB) analysis of MYPT3 polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:A2780 whole cell lysate LaneC:HCT116 whole cell lysate LaneD:H9C2 whole cell lysate LaneE:CT-26 whole cell lysate