P116 Rip polyclonal antibody
Product Specifications
Background
Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.
Product Name Alternative
Myosin phosphatase Rho-interacting protein; M-RIP; Rho-interacting protein 3; RIP3; p116Rip; MPRIP
Swiss Prot
Q6WCQ1
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic peptide, corresponding to Human p116 Rip.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB:1:500~1:1000 IHC:1:50~1:200
Purity
The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 116 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P116 Rip polyclonal antibody detects endogenous levels of p116 Rip protein.
Applications Notes
Western blot (WB) analysis of p116 Rip polyclonal antibody at 1:500 dilution LaneA:PC12 whole cell lysate LaneB:MEF whole cell lysate LaneC:A549 whole cell lysate LaneD:A2780 whole cell lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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