ZAC polyclonal antibody

Product Specifications

Background

Pleiomorphic adenoma gene (PLAG1) encodes a zinc finger protein and is the target gene for pleiomorphic adenomas of the salivary gland. The PLAG family of zinc finger proteins include PLAG1, ZAC1 and PLAG-like 2 (PLAGL2) . ZAC1, also known as PLAGL1, concomitantly controls apoptosis and cell cycle arrest through separate pathways. ZAC1 also acts as a positive or negative transcriptional cofactor for nuclear receptors, depending on the expression of functional p53. ZAC1 is broadly expressed in embryo, with highest expression in the liver primordium, the umbilical region and the neural tube. PLAGL1 is also expressed in normal mammary gland. PLAGL2 functions as a positive regulator of transcription and localizes to the nucleus. PLAGL2 and ZAC1 bind to the DNA consensus sequence ACGGGGGCCCCTTTA. PLAGL2 is ubiquitously expressed with particular abundance in spleen, lung and testis where it may be involved in cell cycle arrest and apoptosis of tumor cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Zinc finger protein PLAGL1; Lost on transformation 1; LOT-1; Pleiomorphic adenoma-like protein 1; Tumor suppressor ZAC; PLAGL1; LOT1; ZAC

Swiss Prot

Q9UM63

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to Human ZAC.

Conjugation

Unconjugated

Applications

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 51 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ZAC polyclonal antibody detects endogenous levels of ZAC protein.

Applications Notes

Western blot (WB) analysis of ZAC polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:U-87MG whole cell lysate LaneC:PC12 whole cell lysate LaneD:CT26 whole cell lysate