USP32 polyclonal antibody

Product Specifications

Background

Ubiquitin-specific protease (USP6) is a 1,406 amino acid protein that is very hydrophilic and has 2 charge clusters that are characteristic of nucleic acidbinding regions. The USP6 gene is oncogenic and originated from the chimeric fusion of two genes: USP32 and TBC1D3. USP32 is an ancient, highly conserved gene, whereas the TBC1D3 gene came from a relatively recent segmental duplication. This duplication is absent in most other mammals and shows rapid amplification and propagation throughout the primate lineage. The chimeric USP6, however, only exists in the hominoid lineage of primates, so it may have contributed to hominoid speciation. USP6 is testis-specific, suggesting an implication in the emergence of reproductive barriers.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ubiquitin carboxyl-terminal hydrolase 32; Deubiquitinating enzyme 32; Renal carcinoma antigen NY-REN-60; Ubiquitin thioesterase 32; Ubiquitin-specific-processing protease 32; USP32; USP10

Swiss Prot

Q8NFA0

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to Human USP32.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 182 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

USP32 polyclonal antibody detects endogenous levels of USP32 protein.

Applications Notes

Western blot (WB) analysis of USP32 polyclonal antibody at 1:500 dilution LaneA:A549 whole cell lysate LaneB:MCF-7 whole cell lysate LaneC:PC12 whole cell lysate LaneD:AML-12 whole cell lysate