ATG4C polyclonal antibody
Product Specifications
Background
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II) . Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE) -conjugated form, and has the capacity for the binding to autophagosomes.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
APG4 autophagy 4 homolog C (S. cerevisiae) ; APG4 autophagy 4 homolog C; APG4 C; APG4C; ATG 4C; ATG4 autophagy related 4 homolog C (S. cerevisiae) ; Atg4c; ATG4C; AUT (S. cerevisiae) like 1; cysteine endopeptidase; AUT like 1; cysteine endopeptidase (S. cerevisiae) ; AUT like 1; cysteine endopeptidase; AUT like 3 cysteine endopeptidase; AUT-like 3 cysteine endopeptidase; AUTL1; AUTL3; Autophagin 3; Autophagin-3; Autophagy related cysteine endopeptidase 3; Autophagy related protein 4 homolog C
Swiss Prot
Q96DT6
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to Human ATG4C.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 50 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ATG4C polyclonal antibody detects endogenous levels of ATG4C protein.
Applications Notes
Western blot (WB) analysis of ATG4C polyclonal antibody at 1:500 dilution LaneA:AML-12 whole cell lysate LaneB:The Testis tissue lysate of Rat LaneC:MCF-7 whole cell lysate LaneD:L02 whole cell lysate
Host or Source
Rabbit
Available Sizes
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