ATG4A polyclonal antibody

Autophagy, a process that results in the lysosomal-dependent degradation of cytosolic compartments, is carried out by the autophagosome, which is a double-membrane vesicle whose formation is catalyzed by several autophagy-related gene (Atg) proteins. Atg4a (ATG4 autophagy related 4 homolog A), also known as APG4A or AUTL2, is a 398 amino acid protein that localizes to the cytoplasm and belongs to the peptidase C54 family. Expressed in a variety of tissues, including brain, skeletal muscle and fetal liver, Atg4a functions as a cysteine protease that cleaves the C-terminal part of target proteins, such as GABARAP and MAP1LC3, and plays an essential role in autophagy. Atg4a exists as multiple alternatively spliced isoforms and is functionally inhibited by N-ethylmaleimide.

Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae) ; ATG4A; ATG4A_HUMAN; Atg4al; AUT like 2 cysteine endopeptidase; AUT-like 2 cysteine endopeptidase; Autl2; Autophagin 2; Autophagin-2; Autophagy related 4A cysteine peptidase; Autophagy related cysteine endopeptidase 2; Autophagy related protein 4 homolog A; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; AV169859; Cysteine protease ATG4A; hAPG4A

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of ATG4A polyclonal antibody at 1:500 dilution LaneA:HCT116 whole cell lysate LaneB:HepG2 whole cell lysate LaneC:CT26 whole cell lysate LaneD:PC12 whole cell lysate