CEP76 polyclonal antibody

Product Specifications

Background

Centrosomes are the major microtubule-organizing centers of mammalian cells. They are composed of a centriole pair and surrounding microtubule-nucleating material termed pericentriolar material (PCM) . Bipolar mitotic spindle assembly relies on two intertwined processes: centriole duplication and centrosome maturation. Failure to properly orchestrate centrosome duplication and maturation is subsequently linked to spindle defects, which can result in aneuploidy and promote cancer progression. The CEP76 (centrosomal protein of 76 kDa) gene encodes a protein of 659 amino acids. The CEP76 protein is homologous among several species including human, mouse, rat and xenopus, suggesting a conserved function.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Centrosomal protein of 76 kDa; Cep76; CEP76; centrosomal protein 76; C18orf9

Swiss Prot

Q8TAP6

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to Human CEP76.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 76 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CEP76 polyclonal antibody detects endogenous levels of CEP76 protein.

Applications Notes

Western blot (WB) analysis of CEP76 polyclonal antibody at 1:500 dilution LaneA:COS-7 whole cell lysate LaneB:PC3 whole cell lysate LaneC:CT26 whole cell lysate LaneD:PC12 whole cell lysate