IARS2 polyclonal antibody

Product Specifications

Background

The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Accurate discrimination of the structurally similar amino acids valine and isoleucine by isoleucyl-tRNA synthetase (IleRS) results, in part, from a hydrolytic editing reaction, which prevents misactivated valine from being stably joined to tRNAIle. IleRS joins Ile to tRNA (Ile) at its synthetic active site and hydrolyzes incorrectly acylated amino acids at its editing active site. A member of the aminoacyl-tRNA synthetase family, human IleRS has been identified as a target of antibodies in the autoimmune disease polymyositis.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Isoleucine--tRNA ligase, mitochondrial; Isoleucyl-tRNA synthetase; IleRS; IleRS

Swiss Prot

Q9NSE4

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to Human IARS2.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 114 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IARS2 polyclonal antibody detects endogenous levels of IARS2 protein.

Applications Notes

Western blot (WB) analysis of IARS2 polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:Hela whole cell lysate LaneC:H446 whole cell lysate LaneD:The Kidney tissue lysate of Mouse LaneE:The Testis tissue lysate of Mouse LaneF:The Kidney tissue lysate of Rat LaneG:The Testis tissue lysate of Rat