MYT1 polyclonal antibody
Product Specifications
Background
Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase; PKMYT1; MYT1
Swiss Prot
Q99640
Reactivity
Human, Mouse
Immunogen
Synthetic peptide, corresponding to Human MYT1.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB:1:500~1:1000 IHC:1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 70, 50 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MYT1 polyclonal antibody detects endogenous levels of MYT1 protein.
Applications Notes
Western blot (WB) analysis of MYT1 polyclonal antibody at 1:500 dilution LaneA:The Liver tissue lysate of Rat LaneB:The Muscle tissue lysate of Rat
Host or Source
Rabbit
Available Sizes
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