E Cadherin (Phospho-S844) polyclonal antibody

Product Specifications

Background

Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cadherin-1; Cadherin1; Cadherin 1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; CDH1; CDHE; UVO

Swiss Prot

P12830

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human E Cadherin around the phosphorylation site of Serine 844.

Conjugation

Unconjugated

Related Pathways

Neuronal and Glial Cell Marker Atlas, Contribution of ECM and Cytoskeletal Factors to EMT

Applications

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 120 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

E Cadherin (Phospho-S844) polyclonal antibody detects endogenous levels of E Cadherin protein only when phosphorylated at Ser844.

Applications Notes

Western blot (WB) analysis of E Cadherin (Phospho-S844) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:HepG2 whole cell lysate LaneC:A549 whole cell lysate LaneD:PC12 whole cell lysate