USP28 (Phospho-S714) polyclonal antibody
Product Specifications
Background
The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001) .
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Ubiquitin carboxyl-terminal hydrolase 28; Deubiquitinating enzyme 28; Ubiquitin thioesterase 28; Ubiquitin-specific-processing protease 28; USP28; KIAA1515
Swiss Prot
Q96RU2
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human USP28 around the phosphorylation site of Serine 714.
Conjugation
Unconjugated
Applications
WB
Dilution
WB:1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 122 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
USP28 (Phospho-S714) polyclonal antibody detects endogenous levels of USP28 protein only when phosphorylated at Ser714.
Applications Notes
Western blot (WB) analysis of USP28 (Phospho-S714) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:A549 whole cell lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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