NACA1 (Phospho-S43) polyclonal antibody
Product Specifications
Background
This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC) . This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
HSD48; NAC-alpha; NACA1; skNAC
Swiss Prot
Q13765
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human NACA1 around the phosphorylation site of Serine 43.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB:1:500~1:1000 IHC:1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 40 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
NACA1 (Phospho-S43) polyclonal antibody detects endogenous levels of NACA1 protein only when phosphorylated at Ser43.
Applications Notes
Western blot (WB) analysis of NACA1 (Phospho-S43) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:Jurkat whole cell lysate LaneC:NIH3T3 whole cell lysate LaneD:CT26 whole cell lysate LaneE:H9C2 whole cell lysate LaneF:PC12 whole cell lysate
Host or Source
Rabbit
Available Sizes
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