NACA1 (Phospho-S43) polyclonal antibody

Product Specifications

Background

This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC) . This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

HSD48; NAC-alpha; NACA1; skNAC

Swiss Prot

Q13765

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human NACA1 around the phosphorylation site of Serine 43.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 40 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

NACA1 (Phospho-S43) polyclonal antibody detects endogenous levels of NACA1 protein only when phosphorylated at Ser43.

Applications Notes

Western blot (WB) analysis of NACA1 (Phospho-S43) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:Jurkat whole cell lysate LaneC:NIH3T3 whole cell lysate LaneD:CT26 whole cell lysate LaneE:H9C2 whole cell lysate LaneF:PC12 whole cell lysate