Cyclin D1 (Phospho-T288) polyclonal antibody

Product Specifications

Background

Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene; CCND1; BCL1; PRAD1

Swiss Prot

P24385

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Cyclin D1 around the phosphorylation site of Threonine 288.

Conjugation

Unconjugated

Applications

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 33 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cyclin D1 (Phospho-T288) polyclonal antibody detects endogenous levels of Cyclin D1 protein only when phosphorylated at Thr288.

Applications Notes

Western blot (WB) analysis of Cyclin D1 (Phospho-T288) polyclonal antibody at 1:500 dilution LaneA:HCT116 whole cell lysate

Host or Source

Rabbit

Available Sizes

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