BIK (Phospho-S35) polyclonal antibody

Product Specifications

Background

Bik/Nbk (Bcl-2-interacting killer/natural born killer) is a potent pro-apoptotic protein belonging to a group of Bcl-2 family members that includes Bad, Bid, Bim, Hrk, and Noxa, containing a BH3 domain but lacking other conserved domains, BH1 or BH2. Functionally, Bik is able to bind to and antagonize anti-apoptotic Bcl-2 family members including Bcl-2, Bcl-xL, and viral homologs E1B-19K and EBV-BHFR1. The BH3 domain of Bik is essential for its apoptotic activity and interaction with survival proteins. Phosphorylation of Bik is correlated with an increase in its pro-apoptotic activity.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Bcl-2-interacting killer; Apoptosis inducer NBK; BIP1; BP4; BIK; NBK

Swiss Prot

Q13323

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human BIK around the phosphorylation site of Serine 35.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 18 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

BIK (Phospho-S35) polyclonal antibody detects endogenous levels of BIK protein only when phosphorylated at Ser35.

Applications Notes

Western blot (WB) analysis of BIK (Phospho-S35) polyclonal antibody at 1:500 dilution LaneA:The Embryo tissue lysate of Mouse LaneB:The Ovary tissue lysate of Rat LaneC:HEK293T starved with EGF whole cell lysate LaneD:HEK293T whole cell lysate