Acinus (Phospho-S1180) polyclonal antibody

Acinus (apoptotic chromatin condensation inducer in the nucleus) is a caspase substrate that has been implicated in nuclear changes during apoptosis. Chromatin condensation and DNA fragmentation are both nuclear morphological features associated with apoptosis. Acinus is expressed in different isoforms (L, S, S') most likely generated by alternative splicing. During apoptosis Acinus is cleaved by caspase-3 to generate a 23 kDa fragment that was reported to induce chromatin condensation. Acinus has been identified to be a component of the spliceosome complex, ASAP, suggesting a role in pre-mRNA processing. Down regulation of Acinus by RNA interference inhibits cell growth (5) . This study also found that loss of Acinus inhibits DNA fragmentation but not chromatin condensation during apoptosis.

Apoptotic chromatin condensation inducer in the nucleus; Acinus; ACIN1; ACINUS; KIAA0670; SR-related and CTD-associated factor 4; CTD-binding SR-like protein RA4; Splicing factor, arginine/serine-rich 15; SCAF4; KIAA1172; SFRS15; Arginine/serine-rich protein PNISR; PNN-interacting serine/arginine-rich protein; SR-related protein; SR-rich protein; Serine/arginine-rich-splicing regulatory protein 130; SRrp130; Splicing factor, arginine/serine-rich 130; Splicing factor, arginine/serine-rich 18; PNISR; C6orf111; SFRS18; SRRP130; HSPC261; HSPC306; RNA-binding protein 39; CAPER alpha; CAPERalpha; Hepatocellular carcinoma protein 1; RNA-binding motif protein 39; RNA-binding region-containing protein 2; Splicing factor HCC1; RBM39; HCC1; RNPC2

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of Acinus (Phospho-S1180) polyclonal antibody at 1:500 dilution LaneA:A549 whole cell lysate LaneB:Hela whole cell lysate LaneC:Jurkat whole cell lysate LaneD:NIH3T3 whole cell lysate LaneE:H9C2 whole cell lysate LaneF:PC12 whole cell lysate