Vimentin (Phospho-S39) polyclonal antibody

Product Specifications

Background

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Vimentin; VIM

Swiss Prot

P08670

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Vimentin around the phosphorylation site of Serine 39.

Conjugation

Unconjugated

Related Pathways

Contribution of ECM and Cytoskeletal Factors to EMT

Applications

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 57 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Vimentin (Phospho-S39) polyclonal antibody detects endogenous levels of Vimentin protein only when phosphorylated at Ser39.

Applications Notes

Western blot (WB) analysis of Vimentin (Phospho-S39) polyclonal antibody at 1:500 dilution LaneA:PMVEC whole cell lysate LaneB:A2780 whole cell lysate LaneC:3T3-L1 whole cell lysate