HP1 alpha (Phospho-S92) polyclonal antibody

Product Specifications

Background

Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and contain a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha; HP1α; CBX5; HP1A

Swiss Prot

P45973

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human HP1 alpha around the phosphorylation site of Serine 92.

Conjugation

Unconjugated

Related Pathways

Examples of Crosstalk Between Post-translational Modifications, DNA Methylation

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 25 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HP1 alpha (Phospho-S92) polyclonal antibody detects endogenous levels of HP1 alpha protein only when phosphorylated at Ser92.

Applications Notes

Western blot (WB) analysis of HP1 alpha (Phospho-S92) polyclonal antibody at 1:500 dilution LaneA:PC3 whole cell lysate LaneB:The Heart tissue lysate of Mouse LaneC:The Brain tissue lysate of Mouse LaneD:The Heart tissue lysate of Rat