CD45 (Phospho-Y1216) polyclonal antibody
Product Specifications
Background
The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing. The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates. CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling. Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; PTPRC; CD45
Swiss Prot
P08575
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human CD45 around the phosphorylation site of Tyrosine 1216.
Conjugation
Unconjugated
Applications
WB
Dilution
WB:1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 180 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CD45 (Phospho-Y1216) polyclonal antibody detects endogenous levels of CD45 protein only when phosphorylated at Tyr1216.
Applications Notes
Western blot (WB) analysis of CD45 (Phospho-Y1216) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:MCF-7 whole cell lysate LaneC:PC12 whole cell lysate LaneD:AML-12 whole cell lysate
Host or Source
Rabbit
Available Sizes
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