INOS (Phospho-Y151) polyclonal antibody

Product Specifications

Background

Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Nitric oxide synthase, inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; Short name=iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase; NOS 2; NOS2A; Inos

Swiss Prot

P35228

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human iNOS around the phosphorylation site of Tyrosine 151.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 130 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

INOS (Phospho-Y151) polyclonal antibody detects endogenous levels of iNOS protein only when phosphorylated at Tyr151.

Applications Notes

Western blot (WB) analysis of iNOS (Phospho-Y151) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:Jurkat whole cell lysate LaneC:MCF-7 whole cell lysate