LAT (Phospho-Y161) polyclonal antibody

Product Specifications

Background

LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling. Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets. The phosphorylation of LAT at Tyr171 and 191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38; LAT

Swiss Prot

O43561

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human LAT around the phosphorylation site of Tyrosine 161.

Conjugation

Unconjugated

Applications

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 36 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

LAT (Phospho-Y161) polyclonal antibody detects endogenous levels of LAT protein only when phosphorylated at Tyr161.

Applications Notes

Western blot (WB) analysis of LAT (Phospho-Y161) polyclonal antibody at 1:500 dilution LaneA:The Heart tissue lysate of Rat LaneB:The Lung tissue lysate of Rat LaneC:The Heart tissue lysate of Mouse LaneD:The Lung tissue lysate of Mouse LaneE:HEK293T whole cell lysate LaneF:Jurkat whole cell lysate