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PKC beta (Phospho-S661) polyclonal antibody

Product Specifications

Background

PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ) . Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+. On the other hand, nPKC δ and ε, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Protein kinase C beta type; PKC-B; PKC-beta; PKCB; PRKCB1; PRKCB

Swiss Prot

P05771

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human PKC beta around the phosphorylation site of Serine 661.

Conjugation

Unconjugated

Related Pathways

Phospholipase Signaling, G Protein-coupled Receptors Signaling to MAPK Erk

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PKC beta (Phospho-S661) polyclonal antibody detects endogenous levels of PKC beta protein only when phosphorylated at Ser661.

Applications Notes

Western blot (WB) analysis of PKC beta (Phospho-S661) polyclonal antibody at 1:500 dilution LaneA:The Brain tissue lysate of Mouse

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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