MKK5 (Phospho-S311) polyclonal antibody
Product Specifications
Background
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs) . The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAPKK 5; MAPK/ERK kinase 5; MEK 5; MAP2K5; MEK5, MKK5, PRKMK5
Swiss Prot
Q13163
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human MKK5 around the phosphorylation site of Serine 311.
Conjugation
Unconjugated
Applications
WB
Dilution
WB:1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 50 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MKK5 (Phospho-S311) polyclonal antibody detects endogenous levels of MKK5 protein only when phosphorylated at Ser311.
Applications Notes
Western blot (WB) analysis of MKK5 (Phospho-S311) polyclonal antibody at 1:500 dilution LaneA:The Heart tissue lysate of Mouse LaneB:The Heart tissue lysate of Rat LaneC:H1792 whole cell lysate LaneD:MCF-7 whole cell lysate
Host or Source
Rabbit
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