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FOXO3 (Phospho-S315) polyclonal antibody

Product Specifications

Background

FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation.FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3; FKHRL1; FOXO3A

Swiss Prot

O43524

Reactivity

Human, Mouse

Immunogen

Synthetic phosphopeptide derived from human FOXO3 around the phosphorylation site of Serine 315.

Conjugation

Unconjugated

Related Pathways

Insulin &Glucose Signaling

Applications

WB

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 82, 97 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

FOXO3 (Phospho-S315) polyclonal antibody detects endogenous levels of FOXO3 protein only when phosphorylated at Ser315.

Applications Notes

Western blot (WB) analysis of FOXO3 (Phospho-S315) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:NIH3T3 whole cell lysate

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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