CDC25A (Phospho-S82) polyclonal antibody

Product Specifications

Background

Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A; CDC25A

Swiss Prot

P30304

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human CDC25A around the phosphorylation site of Serine 82.

Conjugation

Unconjugated

Related Pathways

G Protein-coupled Receptors Signaling to MAPK Erk

Applications

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CDC25A (Phospho-S82) polyclonal antibody detects endogenous levels of CDC25A protein only when phosphorylated at Ser82.

Applications Notes

Western blot (WB) analysis of CDC25A (Phospho-S82) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:HCT116 whole cell lysate LaneC:NIH3T3 whole cell lysate LaneD:PC12 whole cell lysate