Caveolin 2 (Phospho-Y19) polyclonal antibody

Product Specifications

Background

The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homo-oligomers that interact with cholesterol and other lipids. Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease. Caveolins interact with multiple signaling molecules such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS. It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins such as GRB7. Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Caveolin2; CAV2; Caveolin 2

Swiss Prot

P51636

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Caveolin 2 around the phosphorylation site of Tyrosine 19.

Conjugation

Unconjugated

Related Pathways

ErbB HER Signaling

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 25 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Caveolin 2 (Phospho-Y19) polyclonal antibody detects endogenous levels of Caveolin 2 protein only when phosphorylated at Tyr19.

Applications Notes

Western blot (WB) analysis of Caveolin 2 (Phospho-Y19) polyclonal antibody at 1:500 dilution LaneA:The Spleen tissue lysate of Mouse LaneB:A549 whole cell lysate LaneC:MCF-7 whole cell lysate