ENOS (Phospho-S615) polyclonal antibody

Product Specifications

Background

NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS) ; inducible NOS (iNOS) or macrophage NOS (mNOS) ; and endothelial NOS (eNOS) .eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; NOS3

Swiss Prot

P29474

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Serine 615.

Conjugation

Unconjugated

Related Pathways

Hypoxia Signaling, Tumor Angiogenesis

Applications

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 140 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ENOS (Phospho-S615) polyclonal antibody detects endogenous levels of eNOS protein only when phosphorylated at Ser615.

Applications Notes

Western blot (WB) analysis of eNOS (Phospho-S615) polyclonal antibody at 1:500 dilution LaneA:K562 whole cell lysate LaneB:The Spleen tissue lysate of Mouse LaneC:The Spleen tissue lysate of Rat