P57 Kip2 (Phospho-T310) polyclonal antibody

Product Specifications

Background

P57 / Kip 2 (or CDKN 1C) is a potent tight binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. The gene encoding human p57 / Kip 2 is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilm's tumor, and Beckwith Wiedemann syndrome (BWS, a cancer syndrome) making it a tumor suppressor candidate. BWS is characterized by numerous growth abnormalities and an increased risk of childhood tumors. Several types of childhood tumors including Wilms' tumor, adrenocortical carcinoma and rhabdomyosarcoma display a specific loss of maternal 11p15 alleles, suggesting that genomic imprinting plays an important part. This region also contains two other imprinted genes, insulin like growth factor II (IGF II) and H19, both of which seem to be implicated in adrenal neoplasms.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cyclin-dependent kinase inhibitor 1C; Cyclin-dependent kinase inhibitor p57; p57Kip2; CDKN1C; KIP2

Swiss Prot

P49918

Reactivity

Human, Mouse

Immunogen

Synthetic phosphopeptide derived from human p57 Kip2 around the phosphorylation site of Threonine 310.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P57 Kip2 (Phospho-T310) polyclonal antibody detects endogenous levels of p57 Kip2 protein only when phosphorylated at Thr310.

Applications Notes

Western blot (WB) analysis of p57 Kip2 (Phospho-T310) polyclonal antibody at 1:500 dilution LaneA:K562 whole cell lysate LaneB:Hela whole cell lysate LaneC:HEK293T whole cell lysate