Histone Deacetylase 3 (Phospho-S424) polyclonal antibody

Product Specifications

Background

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone deacetylase 3; HD3; RPD3-2; SMAP45; HDAC3

Swiss Prot

O15379

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Histone Deacetylase 3 around the phosphorylation site of Serine 424.

Conjugation

Unconjugated

Related Pathways

Notch Signaling, Nuclear Receptor Signaling, Epigenetic Writers and Erasers of Histone H3

Applications

WB IHC IF

Dilution

WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 49 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Histone Deacetylase 3 (Phospho-S424) polyclonal antibody detects endogenous levels of Histone Deacetylase 3 protein only when phosphorylated at Ser424.

Applications Notes

Western blot (WB) analysis of Histone Deacetylase 3 (Phospho-S424) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:HGC27 whole cell lysate