FOSB (Phospho-S27) polyclonal antibody
Product Specifications
Background
Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the ‘leucine-zipper’ motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Protein fosB; G0/G1 switch regulatory protein 3; FOSB; G0S3
Swiss Prot
P53539
Reactivity
Human, Mouse
Immunogen
Synthetic phosphopeptide derived from human FOSB around the phosphorylation site of Serine 27.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 36 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
FOSB (Phospho-S27) polyclonal antibody detects endogenous levels of FOSB protein only when phosphorylated at Ser27.
Applications Notes
Western blot (WB) analysis of FOSB (Phospho-S27) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:U-87MG whole cell lysate LaneC:PC3 whole cell lysate
Host or Source
Rabbit
Available Sizes
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