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Cytokeratin 8 (Phospho-S432) polyclonal antibody

Product Specifications

Background

Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; Short name=K8; Type-II keratin Kb8; KRT8; CYK8

Swiss Prot

P05787

Reactivity

Human, Mouse

Immunogen

Synthetic phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of Serine 432.

Conjugation

Unconjugated

Related Pathways

Contribution of Soluble Factors to EMT

Applications

WB IHC IF

Dilution

WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cytokeratin 8 (Phospho-S432) polyclonal antibody detects endogenous levels of Cytokeratin 8 protein only when phosphorylated at Ser432.

Applications Notes

Western blot (WB) analysis of Cytokeratin 8 (Phospho-S432) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:A549 whole cell lysate LaneC:The Lung tissue lysate of Mouse LaneD:The Liver tissue lysate of Mouse LaneE:The Lung tissue lysate of Rat

Host or Source

Rabbit

Available Sizes

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