CD142 (Phospho-S290) polyclonal antibody

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which are involved in a blood coagulation cascade leading to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Coagulation Factor V (Factor V, FV, proaccelerin, labile factor) is a 2196 amino acid, single chain glycoprotein that is cleaved by Thrombin to yield an active, Ca2+-dependent dimer that is essential to the blood coagulation cascade. Together with catalytic Factor Xa and Ca2+ on the surface of platelets or endothelial cells, Factor Va coordinates into a Prothrombinase complex, which mediates proteolysis of Prothrombin into active Thrombin. Tissue factor (TF), also designated coagulation Factor III is a cell surface glycoprotein that enables cells to initiate blood coagulation cascades. It functions as a high-affinity receptor for coagulation Factor VII.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of CD142 (Phospho-S290) polyclonal antibody at 1:500 dilution LaneA:The Brain tissue lysate of Mouse LaneB:The Lung tissue lysate of Mouse LaneC:The Liver tissue lysate of Mouse LaneD:The Liver tissue lysate of Rat